Purified poloxamer 188 may increase tissue oxygenation and thereby reduce inflammation, pain, and the overall duration of such painful episodes in patients with scd. Poloxamer 188 c8h18o3 cid 22895896 structure, chemical names, physical and chemical properties, classification, patents, literature, biological activities. It is available in different grades which vary from liquids to solids. Uejsszhhybhceluhfffaoysan silver sulfadiazine chemical.
Drug development and industrial pharmacy downloaded from. Poloxamers, available also under the trademark pluronics basf, are a class of. Nlcs prepared using tween 80 showed high pdi value and less zeta. To better understand the properties of poloxamers, a reversedphase liquid chromatography rplc method was developed to characterize the ingredients in poloxamers. The average molecular weight is about 8,500 kd fig. Product maximum intake is the sponsor justifies by citing a document produced by the manufacturer, that conducted 6 months oral toxicity studies in rats and dogs. Even though tremendous efforts have been spent to understand the mechanism of poloxamer s protection, the root cause for lot. Characterization of poloxamers by reversedphase liquid. Quantitation of poloxamers in pharmaceutical formulations. Lutrol f68, poloxamer 188, polyethylene glycol block polypropylene glycol block polyethylene glycol. Uejsszhhybhceluhfffaoysan silver sulfadiazine chemical compound1.
Apr 11, 2014 poloxamer 188 p188 is a nonionic amphiphilic copolymer consisting of a central chain of hydrophobic polyoxypropylene flanked at both ends by hydrophilic polyoxyethylene. Differential effects of commercialgrade and purified. Download fulltext pdf download fulltext pdf download fulltext pdf read fulltext. Poloxamer 407188 binary thermosensitive hydrogels as. Poloxamer 188 may thus constitute a useful excipient for intramuscular delivery of naked dna 11. Poloxamer 188, surfactant ld50oralmouse no information available 6 10.
Pluracare f 68 poloxamer 188 6 x 300 1800 8 x 10 80 pluracare f 127 poloxamer 407 12 x 300 3600 7 x 10 70 pluracare l 44 poloxamer 124 12 x 100 1200 4 x 10 40 pluracare l 64 poloxamer 184 18 x 100 1800 4 x 10 40 pluracare f 68 poloxamer 188 18 x 100 1800 8 x 10 80 pluracare f 127 poloxamer 407 40 x 100 4000 7 x 10 70. Poloxamer 188 p188 is a copolymer of polyoxyethylene and polyoxypropylene. Mar 31, 2016 variability in poloxamer 188 p188 raw material, which is routinely used in cell culture media to protect cells from hydrodynamic forces, plays an important role in the process performance. Transfer poloxamer 407 to an amber 220 ml graduated prescription bottle. Download pdf info publication number wo2009055312a1. Poloxamer 188 basf pluronic f68 is widely used as a shear. Over the last years, the number of poorly soluble drugs has significantly increased. Poloxamer, shear protectant, lot variability, analytics poloxamer 188 p188, a watersoluble, synthetic copolymer with hydrophilic as well as lipophilic properties, is widely used as a cell protectant for bubbleinduced shear during cultivation of suspensionadapted mammalian. Skin contact wash off immediately with plenty of water for at least 15 minutes. Kr20079297a thermosensitive injectable formulation. Poloxamer 188 5 mgml or a corresponding volume of pbs, as control, was incubated with serum samples from 17 healthy volunteers and the level of sc5b9 was determined.
Composed of two hydrophilic sidechains attached to a hydrophobic center core 2, its average molecular weight is 8400 daltons. Cell culture performance and impurity levels in poloxamer 188. Modification of acute focal ischemia in rabbits by poloxamer 188. Safety assessment of poloxamers 101, 105, 108, 122, 123. Poloxamer 407 188 binary thermosensitive hydrogels as. Poloxamer 188 p188 is a nonionic block linear copolymer that exhibits rheologic, antithrombotic, antiinflammatory, and cytoprotective activities in various tissue injury models 5. Cell culture medium containing a specific poloxamer 188 lot resulted in an unusual drop in cell growth, viability, and titer during manufacturing runs. We applied the sec method to pharmaceutical products containing 0. The effects of poloxamer 188, a triblock copolymer were studied on nanoparticle morphology. Pdf physicochemical features and transfection properties. Causative factors behind poloxamer 188 pluronic f68. Randomized, controlled trial of rheothrx poloxamer 188 in patients with suspected acute myocardial infarction charles maynard, phd,a robert swenson, md,b joseph a. Duvinage c, millecamps s, sagnier a, guffroy m, sarsat jp, belin v.
Pdf poloxamer 188 is a safe biocompatible polymer that can be used in. Fluorescein isothiocyanate fitc was purchased from sigma st. Poloxamer 188 p188, a watersoluble, synthetic copolymer with hydrophilic as well as lipophilic properties, is widely used as a cell protectant for bubbleinduced shear during cultivation of suspensionadapted mammalian cells for largescale production of proteinbased therapeutics. Preclinical safety results phrixus pharmaceuticals. Poloxamers are also known by the trade names pluronic. Pharmaceutical or cosmetic compositions and methods for their use are provided comprising water and a surfactant polymer system comprising a poloxamer at a concentration of about 0. Pdf poloxamer188 and citicoline provide neuronal membrane. Poloxamer 188 solution 10 %, sterilefiltered, bioreagent. Solution stability of poloxamer 188 under stress conditions.
Causative factors behind poloxamer 188 pluronic f68, flocor. Wo2009055312a1 process for producing a poloxamer gel. Adding poloxamer 407 in pharmaceutical encapsulation e. Us8709385b2 poloxamer foamable pharmaceutical compositions. In the present investigation solid dispersions with poloxamer 188 were prepared by fusion method. Series of nonionic surfactants with the structure hoch 2 ch 2 o a chch 3ch 2 o b ch 2 ch 2 o c h where b is at least 15, a and c are approximately equal and the polyoxyethylene content is 10 to 80% of the total polymer weight. The term poloxamer generically applies to the different triblock copolymers made by varying the lengths of the. Pdf lipid corralling and poloxamer squeezeout in membranes.
Briefly, the terminal free hydroxyl groups of poloxamer 188 molecules were capped with acetyl groups to make them inactive without participating in the ringopening polymerization reaction of. The addition of poloxamer 188 to liquid media increases mammalian cell yield in agitated cultures, possibly due to association of poloxamer 188 with the cell membrane 2. The present invention includes both poloxamer 407 and poloxamer 188, and comprises 15% by weight or less of the mixture of poloxamer 407 and poloxamer 188 relative to the total weight of the injection. Kolliphor p 188 is an poloxamer that enables and enhances a wide range of functional applications. The mechanisms that enable poloxamer drug delivery are responsible for advances in a continually expanding number of treatments, each with their own unique challenges. Return to article details preparation and physicochemical characterization of solid dispersion of irbesartan with poloxamer 188 download download pdf preparation and. Purified poloxamer 188 for treatment of acute vaso. Poloxamer 188 p188 is a triblock copolymer of the form polyethylene oxide polypropylene oxidepolyethylene oxide peoppopeo. Safety assessment of poloxamers 101, 105, 108, 122, 123, 124.
Technical information pluracare lf grades poloxamer. Poloxamers 95 table 2 physical and chemical properties of poloxamers. Poloxamer based binary hydrogels for delivering tramadol hydrochloride. Corresponding dosage forms can then be processed by. Onemonth intravenous toxicity studies of poloxamer 188 in spraguedawley rats and in beagle dogs. Poloxamer 188 plays a critical role in protecting cells during cell culture bioprocessing. Topical formulations of poloxamer based thermoreversible gels are being modified to achieve improved compliance and bioavailability 10. Jun 28, 2004 effect of poloxamer 188 on sc5b9 formation in different sera obtained from healthy subjects. Douglas weaver, md,f for the rheothrx in myocardial infarction study group seattle and vancouver, wash buffalo, n. It is used as an emulsifying agent, solubilizing agent, surfactant, and wetting agent for antibiotics. Pdf enhanced rectal bioavailability of ibuprofen in rats by. Wear an n95 mask when dealing with poloxamer 407 powder. Context sickle cell disease scd can cause severe painful episodes that are often thought to be caused by vasoocclusion.
Modulation of protein adsorption by poloxamer 188 in relation. The word poloxamer was coined by basf inventor, irving schmolka, who received the patent for these materials in 1973. Histogram showing the increase in publications related to the keywords poloxamer hydrogel in science direct database during the past twenty years. Poloxamer was eliminated primarily by renal excretion. Preparation and physicochemical characterization of solid. Injectables comprising poloxamer according to the present invention is not only prolonged the interval retention time secured compared to conventional injectables. Pdf preparation and characterization of celecoxib solid. Hunter, md, phd we studied the effect of a synthetic copolymer surfactant, poloxamer 188, on cerebral blood flow in a rabbit model of focal cerebral ischemia. Poloxamer 188 is a surfactant with hemorheological, antithrombotic, and neutrophilinhibitory properties. Specification for poloxamer 188, nf p1169 item number p1169 item poloxamer 188, nf cas number 9003116 molecular formula hoc 2 h 4 o a c 3 h 6 o b c 2 h 4 o a h molecular weight approx. Disposal considerations waste disposal methods chemical waste generators must determine whether a discarded chemical is classified as a hazardous waste.
Poloxamerbased in situ hydrogels for controlled delivery. Use usp poloxamer liquid rs for scan the region at 05 ppm, and use the calculation poloxamer 124, and use usp poloxamer solid rs for formulas specified below. Poloxamer 407 p407 and poloxamer 188 p188 are the polymers frequently used for prepar ation of thermoreversible gels and enhancement of drug solubility, respectively. Pdf multiblock poloxamer surfactants suppress aggregation. Physical and chemical properties as described by leaf 1967, many poloxamers dissolve in water and form gels when a concentrated aqueous solution is made. Aihua liu, brandon wilcock, laixin wang, scott reuschel.
Poloxamer property 124 188 237 338 407 average 20902360 a76809510 68408830 1270017400a 984014600a molecular weight description colorless liquidb white solid asolid solida white solid ph, 2. Pcl is a biodegradable polyester with good permeability and low toxicity, hence indicated for drugdelivery systems. Theranostics, magnetite, nano spray drying, pcl, poloxamer 188. Mechanism investigation for poloxamer 188 raw material. Poloxamers are nonionic triblock copolymers composed of a central hydrophobic chain of polyoxypropylene polypropylene oxide flanked by two hydrophilic chains of polyoxyethylene polyethylene oxide. The current therapy for these uncomplicated painful episodes includes hydration, oxygen, and analgesics. Pdf effect of poloxamer 188 on in vitro dissolution properties of. Ftir spectra indicate that there is no chemical interaction between irbesartan and poloxamer 188 in a solid dispersion system. The effect of poloxamer188 on neuronal cell recovery from. Nov 17, 2014 poloxamer 188 improves heart functioning in a rodent model of ischemic heart failure.
Pluracare f 68 poloxamer 188 6 x 300 1800 8 x 10 80 pluracare f 127 poloxamer 407 12 x 300 3600 7 x 10 70 pluracare l 44 poloxamer 124 12 x 100 1200 4 x 10 40 pluracare l 64 poloxamer 184 18 x 100 1800 4 x 10 40 pluracare f 68 poloxamer 188 18 x 100 1800 8 x 10 80. Because of the dif1 the average area of the doublet appearing at about 1. This agent has been demonstrated to reduce infarct size and to improve left ventricular function in animal models of myocardial infarction and reperfusion, and recently in a randomized trial of patients receiving thrombolytic therapy for acute myocardial infarction. However, little is known in any quantitative sense of its effect on protein adsorption and aggregation. Poloxamer 188 p188 is a triblock copolymer of the form polyethylene oxidepolypropylene oxidepolyethylene oxide peoppopeo. Pdf randomized, controlled trial of rheothrx poloxamer 188. In formulations that include poorly soluble actives, kolliphor p 188 can improve solubility, absorption and. Hmdb is offered to the public as a freely available resource. The effect of poloxamer 188 on nanoparticle morphology.
Uses of poloxamer uses concentration% fat emulsifier 0. In rats and dogs, the noel of 2500 and 100 mgkgday respectively, was established nonionic surfactants, ed. Manufacturing and controls for kolliphor p 188 bio are designed to provide consistent quality for cell culture applications. Polyethylenepolypropylene glycol test specification min max average molecular weight 7,680 9,510. View of preparation and physicochemical characterization. Download limit exceeded you have exceeded your daily download allowance. F68 was incorporated into pcl matrices as both a pore. Poloxamer 188 emprove expert cas 9003116, 128370 stabilized with 70ppm bht ph eur,nf find msds or sds, a coa, data sheets and more. A randomized, double blind, placebo controlled, dose escalation study of the toxicity and pharmacokinetics of poloxamer 188 rheothrx was conducted in 36 healthy male subjects ages 1935 yr who received iv injections of 1090 mgkghr poloxamer or placebo. Poloxamer 188 is a polydisperse mixture of triblock copolymers. Development of small scale cell culture models for.
For instant and modified release applications, kolliphor p 188 can act as a stabilizer in oral suspensions. Poloxamer 105 benzoate is an ester of poloxamer 105 and benzoic acid and poloxamer 182 dibenzoate is the diester of poloxamer 182 and benzoic acid gottschalck and mcewen 2004. Aihua liu, brandon wilcock, laixin wang, scott reuschel and. Linear aminoterminated poly ethylene glycol methyl ethers mpegnh 2 were purchased from jenkem technology co. Sigmaaldrich offers a number of poloxamer 188 products. Use and redistribution of the data, in whole or in part, for commercial purposes requires explicit permission of the authors and explicit acknowledgment of the source material hmdb and the original publication see the hmdb citing page. An increased level of uptake of plga poloxamer 188 nanoparticles in the. Feb 01, 2010 the amount of incorporated poloxamer component in nanoparticles could be calculated by comparing the absorbance of specific bands for nanoparticles with the reference standard curve.
Identification name poloxamer 188 accession number db133 description. A micellar formulation of amphotericin b amb solubilized with poloxamer 188. Tween 80 showed smaller particle size compared to tween 20 and poloxamer 188. Chemical waste generators must also consult local, regional, and national hazardous waste regulations to ensure complete and accurate classification. Release profiles of ropivacaine from poloxamer 407 p407 and p407poloxamer 188 p188 hydrogels 42.
Pdf poloxamer hydrogels for biomedical applications. The effect of poloxamer 188 on nanoparticle morphology, size. P1161 lc50inhalationrat 320 mgm3 inhalation lc50 rat 4 h product name. Firstaid measures eye contact rinse immediately with plenty of water, also under the eyelids, for at least 15 minutes. Preparation and characterization of cefuroxime axetil solid. Poloxamerbased thermoreversible gel for topical delivery. Poloxamer 188 9003116 ld50dermalrabbit 2000 mgkg product code. Addition of poloxamer 188 led to optimise the tsolygel in order to target a specific range of temperature fig.
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